The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
Identifieur interne : 000B59 ( Main/Exploration ); précédent : 000B58; suivant : 000B60The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
Auteurs : Alex L. Lai [États-Unis] ; Jean K. Millet [États-Unis] ; Susan Daniel [États-Unis] ; Jack H. Freed [États-Unis] ; Gary R. Whittaker [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2017.
Descripteurs français
- KwdFr :
- Calcium (métabolisme), Cellules HEK293, Conformation des protéines, Fragments peptidiques (), Fragments peptidiques (métabolisme), Fusion membranaire (physiologie), Glycoprotéine de spicule des coronavirus (), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Pénétration virale, Similitude de séquences, Séquence d'acides aminés, Séquence nucléotidique, Virus du SRAS (), Virus du SRAS (métabolisme).
- MESH :
- métabolisme : Calcium, Fragments peptidiques, Glycoprotéine de spicule des coronavirus, Virus du SRAS.
- physiologie : Fusion membranaire.
- Cellules HEK293, Conformation des protéines, Fragments peptidiques, Glycoprotéine de spicule des coronavirus, Humains, Pénétration virale, Similitude de séquences, Séquence d'acides aminés, Séquence nucléotidique, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Calcium (metabolism), HEK293 Cells, Humans, Membrane Fusion (physiology), Peptide Fragments (chemistry), Peptide Fragments (metabolism), Protein Conformation, SARS Virus (chemistry), SARS Virus (metabolism), Sequence Homology, Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (metabolism), Virus Internalization.
- MESH :
- chemical , chemistry : Peptide Fragments, Spike Glycoprotein, Coronavirus.
- chemical , metabolism : Calcium, Peptide Fragments, Spike Glycoprotein, Coronavirus.
- chemistry : SARS Virus.
- metabolism : SARS Virus.
- physiology : Membrane Fusion.
- Amino Acid Sequence, Base Sequence, HEK293 Cells, Humans, Protein Conformation, Sequence Homology, Virus Internalization.
Abstract
Coronaviruses (CoVs) are a major infectious disease threat and include the pathogenic human pathogens of zoonotic origin: severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Entry of CoVs into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the CoV fusion peptide (FP) has been disputed. However, most evidence suggests that the domain immediately downstream of the S2′ cleavage site is the FP (amino acids 798–818 SFIEDLLFNKVTLADAGFMKQY for SARS-CoV, FP1). In our previous electron spin resonance spectroscopic studies, the membrane-ordering effect of influenza virus, HIV, and Dengue virus FPs has been consistently observed. In this study, we used this effect as a criterion to identify and characterize the
Url:
DOI: 10.1016/j.jmb.2017.10.017
PubMed: 29056462
PubMed Central: 5705393
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000E73
- to stream Pmc, to step Curation: 000E73
- to stream Pmc, to step Checkpoint: 000700
- to stream PubMed, to step Corpus: 000B10
- to stream PubMed, to step Curation: 000B10
- to stream PubMed, to step Checkpoint: 000A97
- to stream Ncbi, to step Merge: 001C23
- to stream Ncbi, to step Curation: 001C23
- to stream Ncbi, to step Checkpoint: 001C23
- to stream Main, to step Merge: 000B62
- to stream Main, to step Curation: 000B59
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner</title><author><name sortKey="Lai, Alex L" sort="Lai, Alex L" uniqKey="Lai A" first="Alex L." last="Lai">Alex L. Lai</name><affiliation wicri:level="4"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><affiliation wicri:level="4"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><affiliation wicri:level="4"><nlm:aff id="af0015">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Freed, Jack H" sort="Freed, Jack H" uniqKey="Freed J" first="Jack H." last="Freed">Jack H. Freed</name><affiliation wicri:level="4"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name><affiliation wicri:level="4"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29056462</idno><idno type="pmc">5705393</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705393</idno><idno type="RBID">PMC:5705393</idno><idno type="doi">10.1016/j.jmb.2017.10.017</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000E73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E73</idno><idno type="wicri:Area/Pmc/Curation">000E73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E73</idno><idno type="wicri:Area/Pmc/Checkpoint">000700</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000700</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:29056462</idno><idno type="wicri:Area/PubMed/Corpus">000B10</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000B10</idno><idno type="wicri:Area/PubMed/Curation">000B10</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000B10</idno><idno type="wicri:Area/PubMed/Checkpoint">000A97</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000A97</idno><idno type="wicri:Area/Ncbi/Merge">001C23</idno><idno type="wicri:Area/Ncbi/Curation">001C23</idno><idno type="wicri:Area/Ncbi/Checkpoint">001C23</idno><idno type="wicri:doubleKey">0022-2836:2017:Lai A:the:sars:cov</idno><idno type="wicri:Area/Main/Merge">000B62</idno><idno type="wicri:Area/Main/Curation">000B59</idno><idno type="wicri:Area/Main/Exploration">000B59</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner</title><author><name sortKey="Lai, Alex L" sort="Lai, Alex L" uniqKey="Lai A" first="Alex L." last="Lai">Alex L. Lai</name><affiliation wicri:level="4"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><affiliation wicri:level="4"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><affiliation wicri:level="4"><nlm:aff id="af0015">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Freed, Jack H" sort="Freed, Jack H" uniqKey="Freed J" first="Jack H." last="Freed">Jack H. Freed</name><affiliation wicri:level="4"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name><affiliation wicri:level="4"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">Ithaca (New York)</settlement></placeName><orgName type="university">Université Cornell</orgName></affiliation></author></analytic><series><title level="j">Journal of Molecular Biology</title><idno type="ISSN">0022-2836</idno><idno type="eISSN">1089-8638</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Calcium (metabolism)</term><term>HEK293 Cells</term><term>Humans</term><term>Membrane Fusion (physiology)</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (metabolism)</term><term>Protein Conformation</term><term>SARS Virus (chemistry)</term><term>SARS Virus (metabolism)</term><term>Sequence Homology</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Calcium (métabolisme)</term><term>Cellules HEK293</term><term>Conformation des protéines</term><term>Fragments peptidiques ()</term><term>Fragments peptidiques (métabolisme)</term><term>Fusion membranaire (physiologie)</term><term>Glycoprotéine de spicule des coronavirus ()</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Pénétration virale</term><term>Similitude de séquences</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Virus du SRAS ()</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptide Fragments</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Peptide Fragments</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term><term>Fragments peptidiques</term><term>Glycoprotéine de spicule des coronavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fusion membranaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Membrane Fusion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>HEK293 Cells</term><term>Humans</term><term>Protein Conformation</term><term>Sequence Homology</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules HEK293</term><term>Conformation des protéines</term><term>Fragments peptidiques</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Pénétration virale</term><term>Similitude de séquences</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Coronaviruses (CoVs) are a major infectious disease threat and include the pathogenic human pathogens of zoonotic origin: severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Entry of CoVs into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the CoV fusion peptide (FP) has been disputed. However, most evidence suggests that the domain immediately downstream of the S2′ cleavage site is the FP (amino acids 798–818 SFIEDLLFNKVTLADAGFMKQY for SARS-CoV, FP1). In our previous electron spin resonance spectroscopic studies, the membrane-ordering effect of influenza virus, HIV, and Dengue virus FPs has been consistently observed. In this study, we used this effect as a criterion to identify and characterize the <italic>bona fide</italic> SARS-CoV FP. Our results indicate that both FP1 and the region immediately downstream (amino acids 816–835 KQYGECLGDINARDLICAQKF, FP2) induce significant membrane ordering. Furthermore, their effects are calcium dependent, which is consistent with <italic>in vivo</italic> data showing that calcium is required for SARS-CoV S-mediated fusion. Isothermal titration calorimetry showed a direct interaction between calcium cations and both FPs. This Ca<sup>2 +</sup>-dependency membrane ordering was not observed with influenza FP, indicating that the CoV FP exhibits a mechanistically different behavior. Membrane-ordering effects are greater and penetrate deeper into membranes when FP1 and FP2 act in a concerted manner, suggesting that they form an extended fusion “platform.”</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="White, J" uniqKey="White J">J. White</name></author><author><name sortKey="Kartenbeck, J" uniqKey="Kartenbeck J">J. Kartenbeck</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A. Helenius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sieczkarski, S B" uniqKey="Sieczkarski S">S.B. Sieczkarski</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guillen, J" uniqKey="Guillen J">J. Guillén</name></author><author><name sortKey="Perez Berna, A J" uniqKey="Perez Berna A">A.J. Pérez-Berná</name></author><author><name sortKey="Moreno, M R" uniqKey="Moreno M">M.R. Moreno</name></author><author><name sortKey="Villalain, J" uniqKey="Villalain J">J. Villalaín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guillen, J" uniqKey="Guillen J">J. Guillén</name></author><author><name sortKey="Kinnunen, P K J" uniqKey="Kinnunen P">P.K.J. Kinnunen</name></author><author><name sortKey="Villalain, J" uniqKey="Villalain J">J. Villalaín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Madu, I G" uniqKey="Madu I">I.G. Madu</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Licitra, B N" uniqKey="Licitra B">B.N. Licitra</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sainz, B" uniqKey="Sainz B">B. Sainz</name></author><author><name sortKey="Rausch, J M" uniqKey="Rausch J">J.M. Rausch</name></author><author><name sortKey="Gallaher, W R" uniqKey="Gallaher W">W.R. Gallaher</name></author><author><name sortKey="Garry, R F" uniqKey="Garry R">R.F. Garry</name></author><author><name sortKey="Wimley, W C" uniqKey="Wimley W">W.C. Wimley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lorieau, J L" uniqKey="Lorieau J">J.L. Lorieau</name></author><author><name sortKey="Louis, J M" uniqKey="Louis J">J.M. Louis</name></author><author><name sortKey="Bax, A" uniqKey="Bax A">A. Bax</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Bushweller, J H" uniqKey="Bushweller J">J.H. Bushweller</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Chu, V C" uniqKey="Chu V">V.C. Chu</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Basso, L G M" uniqKey="Basso L">L.G.M. Basso</name></author><author><name sortKey="Vicente, E F" uniqKey="Vicente E">E.F. Vicente</name></author><author><name sortKey="Crusca, E" uniqKey="Crusca E">E. Crusca</name></author><author><name sortKey="Cilli, E M" uniqKey="Cilli E">E.M. Cilli</name></author><author><name sortKey="Costa Filho, A J" uniqKey="Costa Filho A">A.J. Costa-Filho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mahajan, M" uniqKey="Mahajan M">M. Mahajan</name></author><author><name sortKey="Bhattacharjya, S" uniqKey="Bhattacharjya S">S. Bhattacharjya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Madu, I G" uniqKey="Madu I">I.G. Madu</name></author><author><name sortKey="Roth, S L" uniqKey="Roth S">S.L. Roth</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A C" uniqKey="Walls A">A.C. Walls</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Frenz, B" uniqKey="Frenz B">B. Frenz</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F. DiMaio</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D. Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, R N" uniqKey="Kirchdoerfer R">R.N. Kirchdoerfer</name></author><author><name sortKey="Cottrell, C A" uniqKey="Cottrell C">C.A. Cottrell</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Pallesen, J" uniqKey="Pallesen J">J. Pallesen</name></author><author><name sortKey="Yassine, H M" uniqKey="Yassine H">H.M. Yassine</name></author><author><name sortKey="Turner, H L" uniqKey="Turner H">H.L. Turner</name></author><author><name sortKey="Corbett, K S" uniqKey="Corbett K">K.S. Corbett</name></author><author><name sortKey="Graham, B S" uniqKey="Graham B">B.S. Graham</name></author><author><name sortKey="Mclellan, J S" uniqKey="Mclellan J">J.S. McLellan</name></author><author><name sortKey="Ward, A B" uniqKey="Ward A">A.B. Ward</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A C" uniqKey="Walls A">A.C. Walls</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Frenz, B" uniqKey="Frenz B">B. Frenz</name></author><author><name sortKey="Snijder, J" uniqKey="Snijder J">J. Snijder</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F. DiMaio</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.-J. Bosch</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D. Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panaretakis, T" uniqKey="Panaretakis T">T. Panaretakis</name></author><author><name sortKey="Kepp, O" uniqKey="Kepp O">O. Kepp</name></author><author><name sortKey="Brockmeier, U" uniqKey="Brockmeier U">U. Brockmeier</name></author><author><name sortKey="Tesniere, A" uniqKey="Tesniere A">A. Tesniere</name></author><author><name sortKey="Bjorklund, A C" uniqKey="Bjorklund A">A.C. Bjorklund</name></author><author><name sortKey="Chapman, D C" uniqKey="Chapman D">D.C. Chapman</name></author><author><name sortKey="Durchschlag, M" uniqKey="Durchschlag M">M. Durchschlag</name></author><author><name sortKey="Joza, N" uniqKey="Joza N">N. Joza</name></author><author><name sortKey="Pierron, G" uniqKey="Pierron G">G. Pierron</name></author><author><name sortKey="Van Endert, P" uniqKey="Van Endert P">P. van Endert</name></author><author><name sortKey="Yuan, J" uniqKey="Yuan J">J. Yuan</name></author><author><name sortKey="Zitvogel, L" uniqKey="Zitvogel L">L. Zitvogel</name></author><author><name sortKey="Madeo, F" uniqKey="Madeo F">F. Madeo</name></author><author><name sortKey="Williams, D B" uniqKey="Williams D">D.B. Williams</name></author><author><name sortKey="Kroemer, G" uniqKey="Kroemer G">G. Kroemer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, M" uniqKey="Gui M">M. Gui</name></author><author><name sortKey="Song, W" uniqKey="Song W">W. Song</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J. Xu</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S. Chen</name></author><author><name sortKey="Xiang, Y" uniqKey="Xiang Y">Y. Xiang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y. Yuan</name></author><author><name sortKey="Cao, D" uniqKey="Cao D">D. Cao</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Ma, J" uniqKey="Ma J">J. Ma</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y. Shi</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinello, J F" uniqKey="Pinello J">J.F. Pinello</name></author><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Cassidy Hanley, D" uniqKey="Cassidy Hanley D">D. Cassidy-Hanley</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author><author><name sortKey="Clark, T G" uniqKey="Clark T">T.G. Clark</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Park, H" uniqKey="Park H">H. Park</name></author><author><name sortKey="White, J M" uniqKey="White J">J.M. White</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Steinhauer, D A" uniqKey="Steinhauer D">D.A. Steinhauer</name></author><author><name sortKey="Wharton, S A" uniqKey="Wharton S">S.A. Wharton</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Meer, G" uniqKey="Van Meer G">G. van Meer</name></author><author><name sortKey="Voelker, D R" uniqKey="Voelker D">D.R. Voelker</name></author><author><name sortKey="Feigenson, G W" uniqKey="Feigenson G">G.W. Feigenson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author><author><name sortKey="Ellena, J F" uniqKey="Ellena J">J.F. Ellena</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takamori, S" uniqKey="Takamori S">S. Takamori</name></author><author><name sortKey="Holt, M" uniqKey="Holt M">M. Holt</name></author><author><name sortKey="Stenius, K" uniqKey="Stenius K">K. Stenius</name></author><author><name sortKey="Lemke, E A" uniqKey="Lemke E">E.A. Lemke</name></author><author><name sortKey="Gronborg, M" uniqKey="Gronborg M">M. Gronborg</name></author><author><name sortKey="Riedel, D" uniqKey="Riedel D">D. Riedel</name></author><author><name sortKey="Urlaub, H" uniqKey="Urlaub H">H. Urlaub</name></author><author><name sortKey="Schenck, S" uniqKey="Schenck S">S. Schenck</name></author><author><name sortKey="Brugger, B" uniqKey="Brugger B">B. Brugger</name></author><author><name sortKey="Ringler, P" uniqKey="Ringler P">P. Ringler</name></author><author><name sortKey="Muller, S A" uniqKey="Muller S">S.A. Muller</name></author><author><name sortKey="Rammner, B" uniqKey="Rammner B">B. Rammner</name></author><author><name sortKey="Grater, F" uniqKey="Grater F">F. Grater</name></author><author><name sortKey="Hub, J S" uniqKey="Hub J">J.S. Hub</name></author><author><name sortKey="De Groot, B L" uniqKey="De Groot B">B.L. De Groot</name></author><author><name sortKey="Mieskes, G" uniqKey="Mieskes G">G. Mieskes</name></author><author><name sortKey="Moriyama, Y" uniqKey="Moriyama Y">Y. Moriyama</name></author><author><name sortKey="Klingauf, J" uniqKey="Klingauf J">J. Klingauf</name></author><author><name sortKey="Grubmuller, H" uniqKey="Grubmuller H">H. Grubmuller</name></author><author><name sortKey="Heuser, J" uniqKey="Heuser J">J. Heuser</name></author><author><name sortKey="Wieland, F" uniqKey="Wieland F">F. Wieland</name></author><author><name sortKey="Jahn, R" uniqKey="Jahn R">R. Jahn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frazier, A A" uniqKey="Frazier A">A.A. Frazier</name></author><author><name sortKey="Roller, C R" uniqKey="Roller C">C.R. Roller</name></author><author><name sortKey="Havelka, J J" uniqKey="Havelka J">J.J. Havelka</name></author><author><name sortKey="Hinderliter, A" uniqKey="Hinderliter A">A. Hinderliter</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Patel, A" uniqKey="Patel A">A. Patel</name></author><author><name sortKey="Mohl, B P" uniqKey="Mohl B">B.-P. Mohl</name></author><author><name sortKey="Roy, P" uniqKey="Roy P">P. Roy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matos, P M" uniqKey="Matos P">P.M. Matos</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Ahn, B" uniqKey="Ahn B">B. Ahn</name></author><author><name sortKey="Lam, W" uniqKey="Lam W">W. Lam</name></author><author><name sortKey="Santos, N C" uniqKey="Santos N">N.C. Santos</name></author><author><name sortKey="Melikyan, G B" uniqKey="Melikyan G">G.B. Melikyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaitseva, E" uniqKey="Zaitseva E">E. Zaitseva</name></author><author><name sortKey="Zaitsev, E" uniqKey="Zaitsev E">E. Zaitsev</name></author><author><name sortKey="Melikov, K" uniqKey="Melikov K">K. Melikov</name></author><author><name sortKey="Arakelyan, A" uniqKey="Arakelyan A">A. Arakelyan</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Villasmil, R" uniqKey="Villasmil R">R. Villasmil</name></author><author><name sortKey="Margolis, L B" uniqKey="Margolis L">L.B. Margolis</name></author><author><name sortKey="Melikyan, G B" uniqKey="Melikyan G">G.B. Melikyan</name></author><author><name sortKey="Chernomordik, L V" uniqKey="Chernomordik L">L.V. Chernomordik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y. Hu</name></author><author><name sortKey="Patel, S" uniqKey="Patel S">S. Patel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, R" uniqKey="Yang R">R. Yang</name></author><author><name sortKey="Prorok, M" uniqKey="Prorok M">M. Prorok</name></author><author><name sortKey="Castellino, F J" uniqKey="Castellino F">F.J. Castellino</name></author><author><name sortKey="Weliky, D P" uniqKey="Weliky D">D.P. Weliky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S. Lee</name></author><author><name sortKey="Saxena, S" uniqKey="Saxena S">S. Saxena</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lou, Y" uniqKey="Lou Y">Y. Lou</name></author><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J" uniqKey="Freed J">J. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seelig, J" uniqKey="Seelig J">J. Seelig</name></author><author><name sortKey="Nebel, S" uniqKey="Nebel S">S. Nebel</name></author><author><name sortKey="Ganz, P" uniqKey="Ganz P">P. Ganz</name></author><author><name sortKey="Bruns, C" uniqKey="Bruns C">C. Bruns</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, J" uniqKey="Yang J">J. Yang</name></author><author><name sortKey="Gabrys, C M" uniqKey="Gabrys C">C.M. Gabrys</name></author><author><name sortKey="Weliky, D P" uniqKey="Weliky D">D.P. Weliky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brennan, S C" uniqKey="Brennan S">S.C. Brennan</name></author><author><name sortKey="Wilkinson, W J" uniqKey="Wilkinson W">W.J. Wilkinson</name></author><author><name sortKey="Tseng, H E" uniqKey="Tseng H">H.-E. Tseng</name></author><author><name sortKey="Finney, B" uniqKey="Finney B">B. Finney</name></author><author><name sortKey="Monk, B" uniqKey="Monk B">B. Monk</name></author><author><name sortKey="Dibble, H" uniqKey="Dibble H">H. Dibble</name></author><author><name sortKey="Quilliam, S" uniqKey="Quilliam S">S. Quilliam</name></author><author><name sortKey="Warburton, D" uniqKey="Warburton D">D. Warburton</name></author><author><name sortKey="Galietta, L J" uniqKey="Galietta L">L.J. Galietta</name></author><author><name sortKey="Kemp, P J" uniqKey="Kemp P">P.J. Kemp</name></author><author><name sortKey="Riccardi, D" uniqKey="Riccardi D">D. Riccardi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhalla, A" uniqKey="Bhalla A">A. Bhalla</name></author><author><name sortKey="Chicka, M C" uniqKey="Chicka M">M.C. Chicka</name></author><author><name sortKey="Tucker, W C" uniqKey="Tucker W">W.C. Tucker</name></author><author><name sortKey="Chapman, E R" uniqKey="Chapman E">E.R. Chapman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brunger, A T" uniqKey="Brunger A">A.T. Brunger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chicka, M C" uniqKey="Chicka M">M.C. Chicka</name></author><author><name sortKey="Hui, E" uniqKey="Hui E">E. Hui</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Chapman, E R" uniqKey="Chapman E">E.R. Chapman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suarez, T" uniqKey="Suarez T">T. Suarez</name></author><author><name sortKey="Gallaher, W R" uniqKey="Gallaher W">W.R. Gallaher</name></author><author><name sortKey="Agirre, A" uniqKey="Agirre A">A. Agirre</name></author><author><name sortKey="Goni, F M" uniqKey="Goni F">F.M. Goni</name></author><author><name sortKey="Nieva, J L" uniqKey="Nieva J">J.L. Nieva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dube, M" uniqKey="Dube M">M. Dubé</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Kielian, M" uniqKey="Kielian M">M. Kielian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Field, K A" uniqKey="Field K">K.A. Field</name></author><author><name sortKey="Aneja, R" uniqKey="Aneja R">R. Aneja</name></author><author><name sortKey="Holowka, D" uniqKey="Holowka D">D. Holowka</name></author><author><name sortKey="Baird, B" uniqKey="Baird B">B. Baird</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Gidwani, A" uniqKey="Gidwani A">A. Gidwani</name></author><author><name sortKey="Brown, H A" uniqKey="Brown H">H.A. Brown</name></author><author><name sortKey="Holowka, D" uniqKey="Holowka D">D. Holowka</name></author><author><name sortKey="Baird, B" uniqKey="Baird B">B. Baird</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Lai, Alex L" sort="Lai, Alex L" uniqKey="Lai A" first="Alex L." last="Lai">Alex L. Lai</name></region><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><name sortKey="Freed, Jack H" sort="Freed, Jack H" uniqKey="Freed J" first="Jack H." last="Freed">Jack H. Freed</name><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B59 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B59 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:5705393
|texte= The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29056462" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |